How to make 30% acrylamide solution

In chemical experiments and industrial applications, acrylamide solution (Acrylamide solution) is one of the common reagents. It is widely used in polymerization, gel electrophoresis, and water treatment and other fields. In particular, 30% acrylamide solution, because of its high concentration, is often used to prepare acrylamide polymer. This article will analyze in detail how to make 30% acrylamide solution to ensure its quality and use effect.

1. Acrylamide solution preparation basis

Before starting to make a 30% acrylamide solution, we first need to understand the basic properties of acrylamide. Acrylamide is a white crystalline solid with high solubility and is capable of forming a clear solution in water. In order to make a 30% acrylamide solution, first prepare the required acrylamide raw material and solvent-water.

It should be noted that acrylamide has certain toxicity, so appropriate safety precautions must be taken during operation, such as wearing gloves, goggles, and operating in a well-ventilated environment.

2. Calculate acrylamide quality

The first step in making a 30% acrylamide solution is to calculate the mass of acrylamide required. A 30% solution means 30 grams of acrylamide per 100 grams of solution. Therefore, if you need to prepare 1 liter of 30% acrylamide solution, first calculate the total mass of the solution:

the mass of 1 liter of water is about 1000 grams. Therefore, the mass of acrylamide in the solution should be: 30% × 1000g = 300g.

Therefore, 300 grams of acrylamide is required to make 1 liter of a 30% acrylamide solution.

3. Dissolved acrylamide

Acrylamide began to dissolve. Since acrylamide is readily soluble in water, only 300 grams of acrylamide need be added to a certain amount of deionized water. To ensure complete dissolution, it is recommended to add about 800 ml of water and stir until the solid is completely dissolved.

If the dissolution is not complete during the stirring process, the solution can be heated slightly, but the temperature should not be too high to avoid the decomposition of acrylamide. In order to speed up the dissolution process, a magnetic stirrer can be used, but be careful not to stir too fast to prevent the solution from splashing.

4. Dilute to exact concentration

When the acrylamide was completely dissolved, the remaining water was added until the solution volume reached 1 liter. At this time, the concentration of acrylamide in the solution was close to 30%. In order to ensure the accuracy of the solution concentration, it can be confirmed by using a concentration meter or by density measurement. If there is a slight deviation in the concentration of the solution, the amount of water can be adjusted to ensure that the exact 30% concentration is achieved.

5. Storage and Use Precautions

After the preparation of the 30% acrylamide solution, special care is required during storage and use. The acrylamide solution should be kept in a cool, dry place away from direct sunlight. If the solution needs to be stored for a long time, it is best to store it in a sealed container with the words "hazardous chemicals" to remind the operator to pay attention to safety.

When using, make sure to wear appropriate protective equipment, such as gloves and goggles, to prevent the acrylamide from contacting the skin or eyes.

6. Acrylamide solution common applications

30% acrylamide solution is widely used in laboratory and industrial fields. For example, in electrophoresis experiments, acrylamide solutions are often used to prepare gels to separate molecules of different sizes; in the field of water treatment, it is used to remove impurities in water, especially in flocculation processes.

Understanding how to make a 30% acrylamide solution is essential for its successful application in various fields. Through the above steps, you can prepare a high-quality solution that meets the requirements.

Summary

How to make a 30% acrylamide solution? It is necessary to accurately calculate the quality and dissolution process of acrylamide to ensure accurate concentration. Acrylamide should be handled in strict accordance with safe operating procedures and appropriate storage measures should be taken. Understanding its scope of application and related considerations can make better use of this chemical for scientific research or industrial operations.